human cscc cell lines a431 Search Results


human cscc cell lines a431  (BioVector Inc)
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BioVector Inc human cscc cell lines a431
GSPs regulated hsa_circ_0070934 expression and the malignant progression of <t>CSCC.</t> <t>A431</t> and SCL-1 cells were treated with different concentrations of GSPs for 24 h. ( A and B ) The expression of hsa_circ_0070934 was measured by qRT-PCR. MTT assay ( C and D ) and colony formation assay ( E ) were used to detect cell viability and the number of colonies to assess cell proliferation. ( F – H ) Flow cytometry was performed to determine the cell cycle process and apoptotic cells. ( I and J ) The numbers of migrated and invaded cells were evaluated by transwell assay. ( K ) The protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3 were examined using WB analysis. * P < 0.05.
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GSPs regulated hsa_circ_0070934 expression and the malignant progression of CSCC. A431 and SCL-1 cells were treated with different concentrations of GSPs for 24 h. ( A and B ) The expression of hsa_circ_0070934 was measured by qRT-PCR. MTT assay ( C and D ) and colony formation assay ( E ) were used to detect cell viability and the number of colonies to assess cell proliferation. ( F – H ) Flow cytometry was performed to determine the cell cycle process and apoptotic cells. ( I and J ) The numbers of migrated and invaded cells were evaluated by transwell assay. ( K ) The protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3 were examined using WB analysis. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

doi: 10.2147/CMAR.S302084

Figure Lengend Snippet: GSPs regulated hsa_circ_0070934 expression and the malignant progression of CSCC. A431 and SCL-1 cells were treated with different concentrations of GSPs for 24 h. ( A and B ) The expression of hsa_circ_0070934 was measured by qRT-PCR. MTT assay ( C and D ) and colony formation assay ( E ) were used to detect cell viability and the number of colonies to assess cell proliferation. ( F – H ) Flow cytometry was performed to determine the cell cycle process and apoptotic cells. ( I and J ) The numbers of migrated and invaded cells were evaluated by transwell assay. ( K ) The protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3 were examined using WB analysis. * P < 0.05.

Article Snippet: Human CSCC cell lines (A431 and SCL-1) and normal epidermal cells (HaCaT) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, MTT Assay, Colony Assay, Flow Cytometry, Transwell Assay

GSPs inhibited CSCC cell progression by regulating hsa_circ_0070934. ( A ) The transfection efficiency of hsa_circ_0070934 overexpression vector was assessed by detecting hsa_circ_0070934 expression in A431 and SCL-1 cells using qRT-PCR. ( B – L ) A431 and SCL-1 cells were transfected with Vector or hsa_circ_0070934 overexpression vector, and then treated with GSPs. Non-transfected cells were used as GSPs group. Non-transfected and non-treated cells were used as control group. ( B ) QRT-PCR was employed to detect hsa_circ_0070934 expression. Cell viability and the number of colonies were determined using MTT assay ( C and D ) and colony formation assay ( E ) to evaluate cell proliferation. ( F – H ) Cell cycle process and apoptotic cells were measured by flow cytometry. ( I – K ) Transwell assay was performed to assess the numbers of migrated and invaded cells. ( L ) WB analysis was utilized to test the protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

doi: 10.2147/CMAR.S302084

Figure Lengend Snippet: GSPs inhibited CSCC cell progression by regulating hsa_circ_0070934. ( A ) The transfection efficiency of hsa_circ_0070934 overexpression vector was assessed by detecting hsa_circ_0070934 expression in A431 and SCL-1 cells using qRT-PCR. ( B – L ) A431 and SCL-1 cells were transfected with Vector or hsa_circ_0070934 overexpression vector, and then treated with GSPs. Non-transfected cells were used as GSPs group. Non-transfected and non-treated cells were used as control group. ( B ) QRT-PCR was employed to detect hsa_circ_0070934 expression. Cell viability and the number of colonies were determined using MTT assay ( C and D ) and colony formation assay ( E ) to evaluate cell proliferation. ( F – H ) Cell cycle process and apoptotic cells were measured by flow cytometry. ( I – K ) Transwell assay was performed to assess the numbers of migrated and invaded cells. ( L ) WB analysis was utilized to test the protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3. * P < 0.05.

Article Snippet: Human CSCC cell lines (A431 and SCL-1) and normal epidermal cells (HaCaT) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, Quantitative RT-PCR, Control, MTT Assay, Colony Assay, Flow Cytometry, Transwell Assay

Hsa_circ_0070934 sponged miR-136-5p to regulate the progression of GSPs-treated CSCC cells. A431 and SCL-1 cells were transfected with Vector, hsa_circ_0070934, hsa_circ_0070934 + NC mimic or hsa_circ_0070934 + miR-136-5p mimic, and then treated with GSPs. ( A ) MiR-136-5p expression was measured by qRT-PCR. MTT assay ( B and C ) and colony formation assay ( D ) were performed to measure cell viability and the number of colonies to evaluate cell proliferation. ( E – G ) Cell cycle process and apoptotic cells were analyzed using flow cytometry. ( H – K ) The numbers of migrated and invaded cells were determined using transwell assay. ( L ) WB analysis was used to measure the protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

doi: 10.2147/CMAR.S302084

Figure Lengend Snippet: Hsa_circ_0070934 sponged miR-136-5p to regulate the progression of GSPs-treated CSCC cells. A431 and SCL-1 cells were transfected with Vector, hsa_circ_0070934, hsa_circ_0070934 + NC mimic or hsa_circ_0070934 + miR-136-5p mimic, and then treated with GSPs. ( A ) MiR-136-5p expression was measured by qRT-PCR. MTT assay ( B and C ) and colony formation assay ( D ) were performed to measure cell viability and the number of colonies to evaluate cell proliferation. ( E – G ) Cell cycle process and apoptotic cells were analyzed using flow cytometry. ( H – K ) The numbers of migrated and invaded cells were determined using transwell assay. ( L ) WB analysis was used to measure the protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3. * P < 0.05.

Article Snippet: Human CSCC cell lines (A431 and SCL-1) and normal epidermal cells (HaCaT) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China).

Techniques: Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, MTT Assay, Colony Assay, Flow Cytometry, Transwell Assay

MiR-136-5p inhibited CSCC cell progression by targeting PRAF2. ( A and B ) The transfection efficiency of pcDNA PRAF2 overexpression vector was evaluated by detecting the mRNA and protein expression of PRAF2 in A431 and SCL-1 cells using qRT-PCR and WB analysis. ( C and D ) A431 and SCL-1 cells were transfected with NC mimic, miR-136-5p mimic, miR-136-5p mimic + pcDNA or miR-136-5p mimic + PRAF2. ( C and D ) The mRNA and protein expression of PRAF2 was measured by qRT-PCR and WB analysis. Cell viability and the number of colonies were analyzed using MTT assay ( E and F ) and colony formation assay ( G ) to assess cell proliferation. ( H – J ) Flow cytometry was utilized to detect cell cycle process and apoptotic cells. ( K – M ) Transwell assay was employed to evaluate the numbers of migrated and invaded cells. ( N ) The protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3 were detected using WB analysis. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

doi: 10.2147/CMAR.S302084

Figure Lengend Snippet: MiR-136-5p inhibited CSCC cell progression by targeting PRAF2. ( A and B ) The transfection efficiency of pcDNA PRAF2 overexpression vector was evaluated by detecting the mRNA and protein expression of PRAF2 in A431 and SCL-1 cells using qRT-PCR and WB analysis. ( C and D ) A431 and SCL-1 cells were transfected with NC mimic, miR-136-5p mimic, miR-136-5p mimic + pcDNA or miR-136-5p mimic + PRAF2. ( C and D ) The mRNA and protein expression of PRAF2 was measured by qRT-PCR and WB analysis. Cell viability and the number of colonies were analyzed using MTT assay ( E and F ) and colony formation assay ( G ) to assess cell proliferation. ( H – J ) Flow cytometry was utilized to detect cell cycle process and apoptotic cells. ( K – M ) Transwell assay was employed to evaluate the numbers of migrated and invaded cells. ( N ) The protein levels of PCNA, c-caspase 3/caspase 3 and MMP-3 were detected using WB analysis. * P < 0.05.

Article Snippet: Human CSCC cell lines (A431 and SCL-1) and normal epidermal cells (HaCaT) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing, Quantitative RT-PCR, MTT Assay, Colony Assay, Flow Cytometry, Transwell Assay

GSPs restrained CSCC tumor growth via regulating the hsa_circ_0070934/miR-136-5p/PRAF2 axis. A431 cells were injected into nude mice, and then the mice were given a gavage of 200 mg/kg GSPs daily (0 mg/kg GSPs was used as control group) when the tumor volume reached about 100 mm 3 . ( A ) Tumor volume was measured every 4 days. ( B and C ) After the tumor was removed, the tumor was photographed and weighted. ( D and E ) The expression of hsa_circ_0070934 and miR-136-5p was measured by qRT-PCR. ( F and G ) The mRNA and protein expression of PRAF2 was determined using qRT-PCR and WB analysis. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

doi: 10.2147/CMAR.S302084

Figure Lengend Snippet: GSPs restrained CSCC tumor growth via regulating the hsa_circ_0070934/miR-136-5p/PRAF2 axis. A431 cells were injected into nude mice, and then the mice were given a gavage of 200 mg/kg GSPs daily (0 mg/kg GSPs was used as control group) when the tumor volume reached about 100 mm 3 . ( A ) Tumor volume was measured every 4 days. ( B and C ) After the tumor was removed, the tumor was photographed and weighted. ( D and E ) The expression of hsa_circ_0070934 and miR-136-5p was measured by qRT-PCR. ( F and G ) The mRNA and protein expression of PRAF2 was determined using qRT-PCR and WB analysis. * P < 0.05.

Article Snippet: Human CSCC cell lines (A431 and SCL-1) and normal epidermal cells (HaCaT) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China).

Techniques: Injection, Control, Expressing, Quantitative RT-PCR